Protein A Antibody Purification Kit for Protein Purification 2μm, 30 mg / mL
1, Description:
BeaverBeads™ Protein A, Protein G are superparamagnetic beads covalently coated with recombinant Protein A or Protein G that contains Fc binding domains for the purification of monoclonal or polyclonal antibodies from cell lysate, blood plasma, ascites and tissue culture supernatant, and for antibody immobilization studies. The one-step solution can reduce the antibody absorption process to 10 minutes and purification process to 30 minutes, yielding antibody with >90% purity.
2, Product Information:
| Product Name |
BeaverBeadsTM Protein A(or A/G)Antibody
Purification Kit
|
|
Protein A(or A/G)for Antibody Purification ①
|
1 mL |
|
Antibody Binding Buffer ②
|
100 mL |
|
Antibody Elution Buffer I(pH4.5)③
|
50 mL |
|
Antibody Elution Buffer II(pH2.0)④
|
50 mL |
|
Antibody Neutrilization buffer ⑤
|
10 mL |
|
Beads Washing Buffer ⑥
|
10 mL |
|
Beads Storage Buffer ⑦
|
10 mL |
|
Beads Regeneration Buffer ⑧
|
10 mL |
|
Storage Conditions
|
2~8℃ for long-term preservation |
|
Shelf life
|
2 year |
3, Feature:
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1. No centrifugation, magnetic separation can be used, and the use of the chromatograph-agarose gel purification system can greatly shorten the operating time
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2. Avoid the error of the packing loss caused by repeated suction fluid
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3. Avoid damage to active protein by mechanical shearing force in centrifugation
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4, FAQ:
Q1: How to improve the binding efficiency of antibody with magnetic beads?
A1: The binding efficiency of antibody with magnetic beads is related to the origin of the antibody and its subtype, refer to the type of antibody and the affinity efficiency of Protein A ligand antibody. (See attached table 1 ). If the antibody subtype has low affinity with Protein A , users can increase incubation time between antibody and beads (30~120 min), increase pH value (8~9) of the combination buffer and decrease ionic strength (25~100 mM NaCl) to increase affinity efficiency, or select the target antibodies with higher affinity ligands (such as Protein G or Protein A/G).
Q2: How to improve the efficiency of antibody elution?
A2: When the high affinity between antibody and Protein A ligand result in antibody low elution efficiency, users can avoid this situation by reducing the pH value (1.9~2.5) of elution buffer, increasing the ionic strength of elution buffer (2~3 M MgCl2) or prolonging the elution time. But pay attention that antibody could easily aggregate under low pH conditions. Users can adjust the pH to neutral immediately with alkaline buffers (such as Tris, HEPES, etc.).
Product Tags:
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2μm 30 mg / mL 1 mL Protein A Antibody Purification Kit For Protein Purification Images
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